Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CREST Double Nickase Plasmid (m): sc-435337-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CREST Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CREST Double Nickase Plasmid (m) and CREST Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ss18l1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CREST Antibody (D-7): sc-515827
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CREST Double Nickase Plasmid (m) | sc-435337-NIC | 20 µg | $410.00 |
Mouse Ss18l1 encodes CREST, a neural-enriched nuclear protein that functions as a transcriptional regulator through interactions with chromatin remodeling and histone deacetylase complexes. CREST contributes to activity-dependent gene expression programs important for neuronal differentiation, dendritic development, and synaptic plasticity, linking it to broader epigenetic control of neurodevelopmental pathways. Altered regulation of CREST-associated transcriptional networks has been implicated in neurological phenotypes, supporting its use in studies of neuronal gene regulation and chromatin-dependent signaling. In experimental systems, Ss18l1/CREST perturbation is commonly leveraged to dissect mechanisms of transcriptional control in neurons and glia.
CREST Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ss18l1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ss18l1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ss18l1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ss18l1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.