COX5b Antibody (C-5): sc-374416
- COX5b Antibody (C-5) is a mouse monoclonal IgG1 κ COX5b antibody provided at 200 µg/ml
- raised against amino acids 35-129 mapping at the C-terminus of COX5b of human origin
- COX5b Antibody (C-5) is recommended for detection of COX5b of mouse, rat, human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-COX5b Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for COX5b Antibody (C-5) for WB and IHC(P) applications. These reagents are now offered in bundles with COX5b Antibody (C-5) (see ordering information below).
COX5b Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects COX5b protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). COX5b Antibody (C-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. COX5b plays a crucial role in cellular respiration as part of the cytochrome c oxidase (COX) complex, which is essential for the final step of the electron transport chain. This complex is located in the inner mitochondrial membrane, where COX5b facilitates the conversion of oxygen and hydrogen into water, thereby driving the proton gradient necessary for ATP synthesis. The structure of the COX complex consists of a dimeric assembly of 13 subunits, with COX5b being one of the key components that adapt to varying oxygen levels. Under high oxygen conditions, the aerobic isoform COX5a is expressed, while COX5b is upregulated in hypoxic conditions, ensuring efficient energy production under fluctuating environmental conditions. This adaptability underscores COX5b′s importance in maintaining cellular energy homeostasis, making anti-COX5b antibody (C-5) an invaluable tool for researchers studying mitochondrial function and respiratory physiology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
COX5b Antibody (C-5) References:
- Effects of a transition from normoxia to anoxia on yeast cytochrome c oxidase and the mitochondrial respiratory chain: implications for hypoxic gene induction. | David, PS. and Poyton, RO. 2005. Biochim Biophys Acta. 1709: 169-80. PMID: 16084486
- Identification of dysregulated genes in cutaneous squamous cell carcinoma. | Dang, C., et al. 2006. Oncol Rep. 16: 513-9. PMID: 16865251
- Regulation of copper homeostasis by micro-RNA in Arabidopsis. | Yamasaki, H., et al. 2007. J Biol Chem. 282: 16369-78. PMID: 17405879
- HIF-1 regulates cytochrome oxidase subunits to optimize efficiency of respiration in hypoxic cells. | Fukuda, R., et al. 2007. Cell. 129: 111-22. PMID: 17418790
- Fine needle aspiration coupled with real-time PCR: a painless methodology to study adaptive functional changes in skeletal muscle. | Guescini, M., et al. 2007. Nutr Metab Cardiovasc Dis. 17: 383-93. PMID: 17482439
- Inverse regulation of the yeast COX5 genes by oxygen and heme. | Hodge, MR., et al. 1989. Mol Cell Biol. 9: 1958-64. PMID: 2546055
- Isoforms of yeast cytochrome c oxidase subunit V affect the binuclear reaction center and alter the kinetics of interaction with the isoforms of yeast cytochrome c. | Allen, LA., et al. 1995. J Biol Chem. 270: 110-8. PMID: 7814361
- Phylogenetic footprinting of the human cytochrome c oxidase subunit VB promoter. | Bachman, NJ., et al. 1996. Arch Biochem Biophys. 333: 152-62. PMID: 8806766
- Structure/function of oxygen-regulated isoforms in cytochrome c oxidase. | Burke, PV. and Poyton, RO. 1998. J Exp Biol. 201: 1163-75. PMID: 9510528
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COX5b Antibody (C-5) | sc-374416 | 200 µg/ml | $322.00 | |||
COX5b Antibody (C-5): m-IgG Fc BP-HRP Bundle | sc-529739 | 200 µg Ab; 10 µg BP | $361.00 | |||
COX5b Antibody (C-5): m-IgGκ BP-HRP Bundle | sc-522731 | 200 µg Ab, 40 µg BP | $361.00 | |||
COX5b Antibody (C-5) AC | sc-374416 AC | 500 µg/ml, 25% agarose | $424.00 | |||
COX5b Antibody (C-5) HRP | sc-374416 HRP | 200 µg/ml | $322.00 | |||
COX5b Antibody (C-5) FITC | sc-374416 FITC | 200 µg/ml | $336.00 | |||
COX5b Antibody (C-5) PE | sc-374416 PE | 200 µg/ml | $349.00 | |||
COX5b Antibody (C-5) Alexa Fluor® 488 | sc-374416 AF488 | 200 µg/ml | $364.00 | |||
COX5b Antibody (C-5) Alexa Fluor® 546 | sc-374416 AF546 | 200 µg/ml | $364.00 | |||
COX5b Antibody (C-5) Alexa Fluor® 594 | sc-374416 AF594 | 200 µg/ml | $364.00 | |||
COX5b Antibody (C-5) Alexa Fluor® 647 | sc-374416 AF647 | 200 µg/ml | $364.00 | |||
COX5b Antibody (C-5) Alexa Fluor® 680 | sc-374416 AF680 | 200 µg/ml | $364.00 | |||
COX5b Antibody (C-5) Alexa Fluor® 790 | sc-374416 AF790 | 200 µg/ml | $364.00 |