CLIC5 Double Nickase Plasmid (h): sc-405918-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLIC5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CLIC5 Double Nickase Plasmid (h) and CLIC5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CLIC5. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLIC5 Double Nickase Plasmid (h) | sc-405918-NIC | 20 µg | $410.00 | |||
CLIC5 Double Nickase Plasmid (h2) | sc-405918-NIC-2 | 20 µg | $410.00 |
CLIC5 (chloride intracellular channel 5) is a member of the CLIC family of metamorphic proteins that can associate with intracellular membranes and the cortical actin network, supporting ion homeostasis and cell architecture. In human tissues, CLIC5 is enriched in specialized epithelia and sensory cells, where it contributes to membrane–cytoskeleton organization, mechanosensory function, and regulated trafficking at apical surfaces. Through these roles, CLIC5 intersects with processes such as cytoskeletal remodeling, cell polarity maintenance, and ion channel regulation that shape epithelial barrier properties and sensory transduction. Altered CLIC5 function and expression have been linked to phenotypes involving inner ear hair cells and kidney glomerular structures, making it relevant for studying mechanisms underlying sensory deficits and proteinuric renal disease pathways.
CLIC5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CLIC5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CLIC5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CLIC5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CLIC5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.