CLC-6 CRISPR/Cas9 KO Plasmid (h): sc-405938
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLC-6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CLC-6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLC-6 CRISPR/Cas9 KO Plasmid (h) | sc-405938 | 20 µg | $397.00 |
Overview
CLCN6 encodes the intracellular chloride/proton exchanger CLC-6, a member of the CLC family that localizes predominantly to endosomal and lysosomal membranes where it contributes to vesicular ion homeostasis. By coupling chloride transport to proton gradients, CLC-6 is implicated in regulation of endosome maturation, lysosomal acidification, and membrane trafficking processes that influence protein sorting and turnover. Disruption of these pathways can alter neuronal and epithelial cell physiology, and genetic variation in CLCN6 has been associated with neurodevelopmental phenotypes and broader lysosome-related dysfunction in human studies. Accordingly, CLCN6 is commonly investigated in the context of endolysosomal biology, cargo recycling, and cellular stress responses linked to altered organelle pH and ionic balance.
CLC-6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CLCN6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CLCN6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CLCN6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CLC-6 protein expression.
This CRISPR knockout system enables efficient generation of CLCN6-deficient cell models for investigation of CLC-6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CLCN6 exon(s) critical for CLC-6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CLCN6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CLC-6 CRISPR/Cas9 KO Plasmid (h) and CLC-6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CLCN6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CLC-6 HDR Plasmid (h) and CLC-6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CLCN6 homology arms to support homology-directed repair at defined CLCN6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.