CIDE-B CRISPR/Cas9 KO Plasmid (h): sc-403808

Overview

CIDEB encodes CIDE-B, a member of the CIDE domain–containing protein family implicated in regulation of lipid droplet dynamics, energy homeostasis, and cell death–associated processes. In human tissues with active lipid handling, CIDE-B contributes to pathways linking neutral lipid storage and turnover with metabolic signaling, including hepatic lipid metabolism and adipocyte-associated programs. Altered CIDEB expression or function has been associated with dysregulated lipid accumulation and metabolic phenotypes relevant to obesity, insulin resistance, and fatty liver–related disease mechanisms. As a result, CIDEB is studied for its role in coordinating lipid droplet biology with stress responses and mitochondrial function in metabolically active cells.

CIDE-B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CIDEB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CIDEB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CIDEB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CIDE-B protein expression.

This CRISPR knockout system enables efficient generation of CIDEB-deficient cell models for investigation of CIDE-B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting CIDEB exon(s) critical for CIDE-B function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple CIDEB genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by CIDE-B CRISPR/Cas9 KO Plasmid (h) and CIDE-B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CIDEB locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by CIDE-B HDR Plasmid (h) and CIDE-B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CIDEB homology arms to support homology-directed repair at defined CIDEB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.