CHD1 Antibody (C-8): sc-271626
- CHD1 Antibody (C-8) is a mouse monoclonal IgG1 κ CHD1 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1500-1709 mapping at the C-terminus of CHD1 of human origin
- CHD1 Antibody (C-8) is recommended for detection of CHD1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CHD1 Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-271626 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CHD1 Antibody (C-8) for WB applications. These reagents are now offered in bundles with CHD1 Antibody (C-8) (see ordering information below).
CHD1 Antibody (C-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects CHD1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CHD1 (C-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CHD1 protein plays a crucial role in chromatin remodeling, functioning as an ATP-utilizing chromatin assembly factor that facilitates proper organization of chromatin structure, which is essential for gene expression regulation and DNA repair processes. CHD1 unique structure includes two chromodomains that work together to bind to methylated histone tails, a mechanism vital for recognizing epigenetic marks and maintaining chromatin integrity. CHD1 gene is located on chromosome 5q21.1 and encodes a protein consisting of 1,709 amino acids, exhibiting a high degree of conservation with mouse counterpart, sharing 95.5% identity. This conservation underscores CHD1 importance in fundamental cellular processes across species, making anti-CHD1 antibody (C-8) an invaluable tool for researchers studying chromatin dynamics and epigenetic regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CHD1 Antibody (C-8) References:
- Molecular evolution of the avian CHD1 genes on the Z and W sex chromosomes. | Fridolfsson, AK. and Ellegren, H. 2000. Genetics. 155: 1903-12. PMID: 10924484
- Sec-dependent and Sec-independent translocation of haloacid dehalogenase Chd1 of Burkholderia cepacia MBA4 in Escherichia coli. | Tsang, JS. and Sze, J. 2002. FEMS Microbiol Lett. 211: 259-64. PMID: 12076822
- Chromatin remodeling protein Chd1 interacts with transcription elongation factors and localizes to transcribed genes. | Simic, R., et al. 2003. EMBO J. 22: 1846-56. PMID: 12682017
- CHD1 associates with NCoR and histone deacetylase as well as with RNA splicing proteins. | Tai, HH., et al. 2003. Biochem Biophys Res Commun. 308: 170-6. PMID: 12890497
- Chd1 chromodomain links histone H3 methylation with SAGA- and SLIK-dependent acetylation. | Pray-Grant, MG., et al. 2005. Nature. 433: 434-8. PMID: 15647753
- Human but not yeast CHD1 binds directly and selectively to histone H3 methylated at lysine 4 via its tandem chromodomains. | Sims, RJ., et al. 2005. J Biol Chem. 280: 41789-92. PMID: 16263726
- The ISWI and CHD1 chromatin remodelling activities influence ADH2 expression and chromatin organization. | Xella, B., et al. 2006. Mol Microbiol. 59: 1531-41. PMID: 16468993
- Analysis of nucleosome repositioning by yeast ISWI and Chd1 chromatin remodeling complexes. | Stockdale, C., et al. 2006. J Biol Chem. 281: 16279-88. PMID: 16606615
- DNA-binding and chromatin localization properties of CHD1. | Stokes, DG. and Perry, RP. 1995. Mol Cell Biol. 15: 2745-53. PMID: 7739555
- CHD1 is concentrated in interbands and puffed regions of Drosophila polytene chromosomes. | Stokes, DG., et al. 1996. Proc Natl Acad Sci U S A. 93: 7137-42. PMID: 8692958
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHD1 Antibody (C-8) | sc-271626 | 200 µg/ml | $322.00 | |||
CHD1 Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-527269 | 200 µg Ab; 10 µg BP | $361.00 | |||
CHD1 Antibody (C-8): m-IgG1 BP-HRP Bundle | sc-532642 | 200 µg Ab; 20 µg BP | $361.00 | |||
CHD1 Antibody (C-8) X | sc-271626 X | 200 µg/0.1 ml | $322.00 | |||
CHD1 Antibody (C-8) AC | sc-271626 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CHD1 Antibody (C-8) HRP | sc-271626 HRP | 200 µg/ml | $322.00 | |||
CHD1 Antibody (C-8) FITC | sc-271626 FITC | 200 µg/ml | $336.00 | |||
CHD1 Antibody (C-8) PE | sc-271626 PE | 200 µg/ml | $349.00 | |||
CHD1 Antibody (C-8) Alexa Fluor® 488 | sc-271626 AF488 | 200 µg/ml | $364.00 | |||
CHD1 Antibody (C-8) Alexa Fluor® 546 | sc-271626 AF546 | 200 µg/ml | $364.00 | |||
CHD1 Antibody (C-8) Alexa Fluor® 594 | sc-271626 AF594 | 200 µg/ml | $364.00 | |||
CHD1 Antibody (C-8) Alexa Fluor® 647 | sc-271626 AF647 | 200 µg/ml | $364.00 | |||
CHD1 Antibody (C-8) Alexa Fluor® 680 | sc-271626 AF680 | 200 µg/ml | $364.00 | |||
CHD1 Antibody (C-8) Alexa Fluor® 790 | sc-271626 AF790 | 200 µg/ml | $364.00 |