Centrin-1 Double Nickase Plasmid (h): sc-404287-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Centrin-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Centrin-1 Double Nickase Plasmid (h) and Centrin-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CETN1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Centrin-1 Antibody (2A6): sc-293494
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Centrin-1 Double Nickase Plasmid (h) | sc-404287-NIC | 20 µg | $410.00 | |||
Centrin-1 Double Nickase Plasmid (h2) | sc-404287-NIC-2 | 20 µg | $410.00 |
CETN1 encodes centrin-1, a calcium-binding EF-hand protein that localizes to centrosomes and associated microtubule-organizing structures, where it supports centriole biogenesis, centrosome duplication, and spindle integrity during cell division. Through these roles, centrin-1 contributes to cell-cycle progression and maintenance of genome stability, linking its regulation to pathways governing mitotic control and DNA damage responses. Aberrant centrosome number and disordered spindle dynamics are common features of chromosomal instability observed across diverse disease contexts, making CETN1 a useful node for mechanistic studies of centrosome-dependent proliferation and stress signaling.
Centrin-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CETN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CETN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CETN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CETN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.