Cdk8 Double Nickase Plasmid (h): sc-400577-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdk8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cdk8 Double Nickase Plasmid (h) and Cdk8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDK8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdk8 Antibody (D-9): sc-13155
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdk8 Double Nickase Plasmid (h) | sc-400577-NIC | 20 µg | $410.00 | |||
Cdk8 Double Nickase Plasmid (h2) | sc-400577-NIC-2 | 20 µg | $410.00 |
CDK8 encodes cyclin-dependent kinase 8, a core component of the Mediator kinase module that phosphorylates transcriptional regulators to modulate RNA polymerase II–dependent gene expression. Cdk8 integrates signals from pathways such as Wnt/β-catenin, TGF-β, and Notch to influence cell-cycle progression, differentiation, and stress-responsive transcriptional programs. Through context-dependent control of enhancer activity and transcription factor turnover, CDK8 contributes to chromatin-associated regulatory networks that shape cell identity. Dysregulated CDK8 activity or expression has been linked to aberrant transcriptional circuitry observed across multiple disease-relevant models, including oncogenic and inflammatory settings.
Cdk8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDK8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDK8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDK8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDK8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.