Cdk5 Double Nickase Plasmid (h): sc-400161-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdk5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cdk5 Double Nickase Plasmid (h) and Cdk5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDK5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdk5 Antibody (J-3): sc-6247
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdk5 Double Nickase Plasmid (h) | sc-400161-NIC | 20 µg | $410.00 | |||
Cdk5 Double Nickase Plasmid (h2) | sc-400161-NIC-2 | 20 µg | $410.00 |
Cyclin-dependent kinase 5 (CDK5) encodes Cdk5, a proline-directed serine/threonine kinase that is activated by non-cyclin cofactors such as p35 (CDK5R1) and p39 (CDK5R2). In human cells, Cdk5 regulates neuronal development, synaptic signaling, cytoskeletal remodeling, vesicle trafficking, and cell-cycle–independent phosphorylation cascades through substrates that intersect MAPK, PI3K/AKT, and stress-response pathways. Dysregulated Cdk5 activity and aberrant substrate phosphorylation have been linked to neurodegenerative processes, including tau pathology, as well as altered migration and survival programs in multiple disease contexts. Because CDK5 influences both signaling dynamics and structural plasticity, it is widely studied in models of neurobiology, DNA damage responses, and pathway cross-talk driven by kinase networks.
Cdk5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDK5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDK5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDK5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDK5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.