CDK11 Double Nickase Plasmid (h): sc-405330-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CDK11 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CDK11 Double Nickase Plasmid (h) and CDK11 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDK19. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdk11 Antibody (8B6): sc-517026
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CDK11 Double Nickase Plasmid (h) | sc-405330-NIC | 20 µg | $410.00 | |||
CDK11 Double Nickase Plasmid (h2) | sc-405330-NIC-2 | 20 µg | $410.00 |
Human CDK19 encodes a cyclin-dependent kinase that functions within the Mediator kinase module to integrate signaling cues with RNA polymerase II–dependent transcription. By phosphorylating transcriptional regulators and influencing promoter-proximal pause release, CDK19 contributes to control of gene expression programs linked to cell-cycle progression, stress responses, and differentiation. Altered activity or expression of Mediator-associated CDKs has been connected to transcriptional rewiring in cancer and other proliferative disorders, making CDK19 a relevant target for dissecting oncogenic and inflammatory signaling networks. CDK11 protein family members are similarly implicated in transcription and cell-cycle–related processes, underscoring the broader importance of CDK-regulated transcriptional control in human biology.
CDK11 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDK19 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDK19. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDK19 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDK19-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.