Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cdc27 Double Nickase Plasmid (h): sc-400789-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdc27 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cdc27 Double Nickase Plasmid (h) and Cdc27 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDC27. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdc27 Antibody (AF3.1): sc-9972
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc27 Double Nickase Plasmid (h) | sc-400789-NIC | 20 µg | $410.00 | |||
Cdc27 Double Nickase Plasmid (h2) | sc-400789-NIC-2 | 20 µg | $410.00 |
Human CDC27 encodes Cdc27, a core tetratricopeptide repeat (TPR) subunit of the anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase that controls ordered proteolysis of key mitotic regulators. By supporting APC/C assembly and co-activator interactions, Cdc27 contributes to timely metaphase-to-anaphase transition, mitotic exit, and maintenance of genome stability through regulated degradation of cyclins and securin. CDC27 function is therefore tightly linked to cell-cycle progression, spindle checkpoint signaling, and ubiquitin-mediated proteostasis. Altered APC/C activity and CDC27 dysregulation have been associated with chromosomal instability and proliferative phenotypes studied in cancer biology and related cell-cycle disorders.
Cdc27 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDC27 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDC27. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDC27 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDC27-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.