Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD44 Double Nickase Plasmid (m): sc-419558-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD44 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD44 Double Nickase Plasmid (m) and CD44 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cd44. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD44 Antibody (DF1485): sc-7297
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD44 Double Nickase Plasmid (m) | sc-419558-NIC | 20 µg | $410.00 |
Cd44 encodes CD44, a transmembrane glycoprotein that functions as a principal receptor for hyaluronan and other extracellular matrix ligands, coordinating cell–cell and cell–matrix adhesion. In mouse cells, CD44 contributes to cytoskeletal remodeling, migration, and proliferation through pathways that interface with ERM proteins, Rho-family GTPases, and growth factor receptor signaling, shaping tissue organization and inflammatory responses. CD44 isoform usage and glycosylation state influence receptor clustering and downstream signaling, affecting leukocyte trafficking and stromal interactions. Dysregulated CD44 activity is frequently studied in contexts of chronic inflammation, fibrosis, and tumor-associated cell motility, making it a common node for mechanistic studies of microenvironment-driven phenotypes.
CD44 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cd44 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cd44. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cd44 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cd44-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.