Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD44 Double Nickase Plasmid (h): sc-400209-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD44 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD44 Double Nickase Plasmid (h) and CD44 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD44. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD44 Antibody (DF1485): sc-7297
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD44 Double Nickase Plasmid (h) | sc-400209-NIC | 20 µg | $410.00 | |||
CD44 Double Nickase Plasmid (h2) | sc-400209-NIC-2 | 20 µg | $410.00 |
CD44 encodes a cell-surface glycoprotein that functions as a principal receptor for hyaluronan and related extracellular matrix ligands, coordinating cell–cell and cell–matrix adhesion. Through interactions with ERM proteins and receptor tyrosine kinase signaling, CD44 contributes to cytoskeletal remodeling, migration, and survival pathways including Rho GTPase signaling and PI3K–AKT and MAPK/ERK cascades. Alternative splicing and dynamic glycosylation generate CD44 isoforms that influence epithelial–mesenchymal plasticity, inflammatory cell trafficking, and tissue remodeling. Dysregulated CD44 expression and isoform usage are frequently studied in cancer invasion and metastasis, stem-like phenotypes, and chronic inflammatory diseases.
CD44 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD44 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD44. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD44 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD44-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.