Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD42d Double Nickase Plasmid (h): sc-404793-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD42d Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD42d Double Nickase Plasmid (h) and CD42d Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GP5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD42d Antibody (G-11): sc-271662
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD42d Double Nickase Plasmid (h) | sc-404793-NIC | 20 µg | $410.00 | |||
CD42d Double Nickase Plasmid (h2) | sc-404793-NIC-2 | 20 µg | $410.00 |
GP5 encodes platelet glycoprotein V (CD42d), a component of the GPIb-IX-V receptor complex on the platelet surface that cooperates with von Willebrand factor binding to mediate platelet tethering under high shear and supports thrombin-dependent platelet activation. Through these interactions, CD42d contributes to hemostatic adhesion, mechanotransduction, and downstream signaling events that coordinate platelet granule release and integrin activation. Altered expression or function of the GPIb-IX-V complex is associated with inherited platelet adhesion defects and can influence thrombotic and bleeding phenotypes, making GP5 a useful locus for dissecting platelet biology and coagulation-linked inflammatory processes.
CD42d Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GP5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GP5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GP5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GP5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.