CD17 Antibody (Huly-m13/H018.3G-6.F5): sc-65253

CD17 Antibody (Huly-m13/H018.3G-6.F5) is a mouse monoclonal IgM antibody that detects CD17 protein of human origin by immunofluorescence (IF) and flow cytometry (FCM). Anti-CD17 antibody (Huly-m13/H018.3G-6.F5) is available as the non-conjugated format. CD17 protein, also known as Lactosylceramide or LacCer, plays a crucial role in cellular interactions and signaling, particularly in immune response. CD17 (Huly-m13/H018.3G-6.F5) antibody targets an intermediate glycosphingolipid derived from higher gangliosides metabolism, primarily localized to sphingolipid-sterol rafts within cell membrane. This localization facilitates clustering of signaling molecules, essential for effective immune cell communication and function. CD17 is expressed in various immune cells, including monocytes, granulocytes, basophils, platelets, and a subset of peripheral B cells, with dynamic regulation - rapidly downregulating on activated granulocytes while upregulating on interleukin-2 activated T lymphocytes. CD17 binds to bacteria, potentially enhancing phagocytosis, and is produced by vascular endothelial cells in response to vascular endothelial growth factor, mediating signaling pathways that promote angiogenesis through PECAM-1 expression. In neuroinflammation, tumor necrosis factor alpha-induced astrogliosis leads to increased intracellular CD17 levels, highlighting involvement in inflammatory response. Dysregulation of glycosphingolipid levels, including CD17, is often associated with cancer, influencing integrin clustering and internalization, which may contribute to tumor progression.

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CD17 Antibody (Huly-m13/H018.3G-6.F5) References:

1. Lactosylceramide mediates shear-induced endothelial superoxide production and intercellular adhesion molecule-1 expression.  |  Yeh, LH., et al. 2001. J Vasc Res. 38: 551-9. PMID: 11740154
2. Lactosylceramide-enriched glycosphingolipid signaling domain mediates superoxide generation from human neutrophils.  |  Iwabuchi, K. and Nagaoka, I. 2002. Blood. 100: 1454-64. PMID: 12149231
3. Lactosylceramide: effect of acyl chain structure on phase behavior and molecular packing.  |  Li, XM., et al. 2002. Biophys J. 83: 1535-46. PMID: 12202378
4. Lactosylceramide recruits PKCalpha/epsilon and phospholipase A2 to stimulate PECAM-1 expression in human monocytes and adhesion to endothelial cells.  |  Gong, N., et al. 2004. Proc Natl Acad Sci U S A. 101: 6490-5. PMID: 15084746
5. Up-regulation of lactosylceramide synthase in MDR1 overexpressing human liver tumour cells.  |  Hummel, I., et al. 2005. FEBS Lett. 579: 3381-4. PMID: 15936020
6. The glycosphingolipid, lactosylceramide, regulates beta1-integrin clustering and endocytosis.  |  Sharma, DK., et al. 2005. Cancer Res. 65: 8233-41. PMID: 16166299
7. Lactosylceramide: lateral interactions with cholesterol.  |  Zhai, X., et al. 2006. Biophys J. 91: 2490-500. PMID: 16829567
8. Lactosylceramide stimulates Ras-GTP loading, kinases (MEK, Raf), p44 mitogen-activated protein kinase, and c-fos expression in human aortic smooth muscle cells.  |  Bhunia, AK., et al. 1996. J Biol Chem. 271: 10660-6. PMID: 8631872
9. Redox-regulated signaling by lactosylceramide in the proliferation of human aortic smooth muscle cells.  |  Bhunia, AK., et al. 1997. J Biol Chem. 272: 15642-9. PMID: 9188453

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD17 Antibody (Huly-m13/H018.3G-6.F5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CD17 Antibody (Huly-m13/H018.3G-6.F5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD17 Antibody (Huly-m13/H018.3G-6.F5) Specifications

Alternate Product Name: CD17

Product Clone Name: Huly-m13/H018.3G-6.F5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM

Amino Acids:

Epitope Target: PBL

Epitope Species: human

Product Reactivity/Detection: CD17

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, FCM

Vialing Information: Each vial contains 100 µg IgM in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CD17 Antibody (Huly-m13/H018.3G-6.F5): N/A