CD137L Double Nickase Plasmid (h): sc-404974-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD137L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD137L Double Nickase Plasmid (h) and CD137L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TNFSF9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD137L Antibody (G-10): sc-398933
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD137L Double Nickase Plasmid (h) | sc-404974-NIC | 20 µg | $410.00 | |||
CD137L Double Nickase Plasmid (h2) | sc-404974-NIC-2 | 20 µg | $410.00 |
TNFSF9 encodes CD137L (4-1BB ligand), a TNF superfamily member expressed on antigen-presenting cells that engages CD137 (TNFRSF9) on activated T cells and NK cells to shape costimulatory signaling. CD137L–CD137 interactions promote immune synapse formation and regulate cytokine production, proliferation, and survival through NF-κB and MAPK-linked signaling programs. Beyond forward signaling into CD137-positive lymphocytes, CD137L can also transmit reverse signals in myeloid cells that influence activation state, differentiation, and inflammatory output. Dysregulated TNFSF9/CD137L activity has been implicated in chronic inflammation and autoimmunity and is frequently examined in tumor immunology, where immune context and checkpoint pathways modulate CD137L-dependent responses.
CD137L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TNFSF9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TNFSF9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TNFSF9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TNFSF9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.