Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CBS CRISPR/Cas9 KO Plasmid (h): sc-400877
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CBS CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CBS genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CBS Antibody (B-4): sc-133154
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CBS CRISPR/Cas9 KO Plasmid (h) | sc-400877 | 20 µg | $397.00 |
Overview
Cystathionine beta-synthase (CBS) is a pyridoxal phosphate–dependent enzyme that catalyzes the condensation of serine and homocysteine to form cystathionine, committing homocysteine to the transsulfuration pathway. By linking one-carbon metabolism to cysteine biosynthesis, CBS influences cellular redox balance through downstream glutathione production and contributes to sulfur amino acid homeostasis. CBS activity also supports endogenous hydrogen sulfide generation, impacting mitochondrial function and stress-responsive signaling. Dysregulation of CBS is associated with hyperhomocysteinemia and inborn errors of sulfur metabolism, and altered CBS expression has been studied in contexts of oxidative stress and metabolic reprogramming relevant to cardiovascular and neurodevelopmental phenotypes.
CBS CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CBS gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CBS together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CBS open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CBS protein expression.
This CRISPR knockout system enables efficient generation of CBS-deficient cell models for investigation of CBS signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CBS exon(s) critical for CBS function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CBS genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CBS CRISPR/Cas9 KO Plasmid (h) and CBS CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CBS locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CBS HDR Plasmid (h) and CBS HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CBS homology arms to support homology-directed repair at defined CBS target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.