CBP20 Double Nickase Plasmid (h): sc-404124-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CBP20 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CBP20 Double Nickase Plasmid (h) and CBP20 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NCBP2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CBP20 Antibody (B-1): sc-137123
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CBP20 Double Nickase Plasmid (h) | sc-404124-NIC | 20 µg | $410.00 | |||
CBP20 Double Nickase Plasmid (h2) | sc-404124-NIC-2 | 20 µg | $410.00 |
NCBP2 encodes CBP20, the small subunit of the nuclear cap-binding complex that recognizes the 7-methylguanosine cap on nascent RNA polymerase II transcripts. Together with NCBP1/CBP80, CBP20 couples cap recognition to co-transcriptional RNA processing, including pre-mRNA splicing, 3′ end formation, RNA export via TREX-dependent pathways, and surveillance mechanisms such as nonsense-mediated mRNA decay. Through these roles, CBP20 helps coordinate gene expression programs that influence cell-cycle control, stress responses, and innate immune signaling linked to RNA metabolism. Dysregulation of cap-dependent RNA processing and export is frequently observed in disease-associated transcriptomic states, making NCBP2 a relevant target for mechanistic studies of RNA biogenesis and nuclear–cytoplasmic transport.
CBP20 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NCBP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NCBP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NCBP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NCBP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.