Anticorpo CBP/KAT3A/CREBBP (C-1) raccomandato per il rilevamento di CBP p265 di origine mouse, rat, human e in WB, IP, IF, IHC(P) and ELISA

Anticorpo CBP/KAT3A/CREBBP (C-1): sc-7300

Anticorpo CBP/KAT3A/CREBBP (C-1) valutazione 4.3 di 5 di 37.
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Schede tecniche

    • Anti-CBP/KAT3A/CREBBP Antibody C-1 è un monoclonale di topo IgG1 κ CBP/KAT3A/CREBBP antibody, citato in 130 pubblicazioni, fornito in 200 µg/ml
    • specific for an epitope mapping between amino acids 2423-2442 at the C-terminus of CBP of human origin (identical to corresponding mouse sequence)
    • CBP/KAT3A/CREBBP Antibody (C-1) é raccomandato per il rilevamento di CBP p265 di origine mouse, rat, human e in WB, IP, IF, IHC(P) and ELISA; anche reattivo con ulteriori specie, incluse e canine, bovine, porcine and avian
    • CBP/KAT3A/CREBBP Antibody (C-1) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • reagente TransCruz per utilizzo in Gel Supershift e ChIP (sc-7300 X, 200 µg/0.1 ml)
    • Contattaci per ricevere un FREE 10 µg sample di CBP/KAT3A/CREBBP (C-1): sc-7300.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CBP/KAT3A/CREBBP Antibody (C-1). This work is in progress.
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Do you have a directly conjugated isotype control for this antibody. I bought sc-7300 AF594 and need a proper control to show that the positive signal is not a non-specific binding

Richiesta da: zhangll
Thank you for your question. The appropriate isotype control for AF594-conjugated sc-7300 is normal mouse IgG1 Alexa Fluor® 594: sc-516620.
Risposta da: Tech Support Europe
Data di pubblicazione: 2020-09-07

I recently purchased this antibody. However, I need to know if this antibody cross-reacts with the closely related p300 protein (KAT3B aka EP300) in human cells. A rapid and accurate response would be greatly appreciated. Thanks, Isaac

Richiesta da: IHilton
Thank you for your question. This mouse monoclonal antibody, CBP (C-1): sc-7300, is specific for CBP (CREB-binding protein). We have not specifically tested this clone for potential cross-reactivity with KAT3B aka EP300, however this antibody has been validated for specificity using our human siRNA for knockdown against the human gene target, CBP siRNA (h): sc-29244.
Risposta da: Technical Support
Data di pubblicazione: 2019-06-24

antibody Cat#sc-7300: how do I get in possession of the 10ug sample as advertised on your website? Thanks

Richiesta da: Seia
Thank you for your question. For a limited time, we are offering a free 10 µg sample of our mouse monoclonal antibody, CBP Antibody (C-1): sc-7300. Please contact our Technical Service Department and we would be happy to arrange a complimentary shipment for you. You may call us toll free at (800) 457-3801 option 2, or email [email protected], or use our live chat!
Risposta da: Technical Support
Data di pubblicazione: 2017-01-25

Is there any data showing this antibody is specific to CBP?

Richiesta da: jenni
Thank you for your question. We have immunofluorescence data with methanol fixed HeLa cells treated with siRNA to show knockdown of signal, confirming specificity to CBP.
Risposta da: Technical Support 13
Data di pubblicazione: 2020-08-10
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Rated 5 di 5 di da Looks visible and great This antibody works well with transfer buffer that included 0.1%SDS and only 10% methanol. Use for incubation at 1:500
Data di pubblicazione: 2018-12-12
Rated 5 di 5 di da Works in ChIP analysis In ChIP analysis, tThis antibody could detect forskolin-dependent binding of CBP to c-Fos promoter in HEK293FT cells.
Data di pubblicazione: 2018-10-29
Rated 1 di 5 di da not working for me in IFA I was unfortunately not successful in getting a signal with 96 well plate probed with recombinant CBP tagged protein O/N at 4C using a fluorescent secondary antibody for 1hr. Antibodies were concentrated as high as 1:50 in PBS-BSA and incubated for 1-2hrs at RT. I used an anti-CBP from a different vendor that gave me a specific signal. This was also tried on fixed cell lines expressing CBP on the surface with negative results as well.
Data di pubblicazione: 2018-10-24
Rated 5 di 5 di da Good Product 2 The antibody worked well at 1:500 dilution using mouse brain tissue extract.
Data di pubblicazione: 2018-08-29
Rated 2 di 5 di da なんとも言えない 過剰発現したものには当たりましたが、内在性のものはdetectできませんでした。当たりが弱い気はしました。
Data di pubblicazione: 2018-07-03
Rated 2 di 5 di da not satisfied for IF I tested this AB in IF (range 1/20 to 1/100) both in cells and brain E16 mouse the signal was really low (PFA fixation in both case)
Data di pubblicazione: 2018-03-22
Rated 2 di 5 di da no good signal in IF I didn't detect good staining signal in 4%PFA fixed embyronic mouse brain(after antigen retrieval)
Data di pubblicazione: 2018-03-07
Rated 3 di 5 di da It works for western blotting. It detected a faint band with the expected size in nuclear extracts of immortalized HFFs, but not in total protein extracts (1:500 in 5% milk in TBS-0.1% Tween overnight at 4C).
Data di pubblicazione: 2018-02-23
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