catalase CRISPR Activation Plasmid (m): sc-419459-ACT

Mouse Cat encodes catalase, a peroxisomal heme enzyme that decomposes hydrogen peroxide into water and oxygen to maintain cellular redox balance. By limiting H2O2 accumulation, catalase shapes reactive oxygen species signaling, protects lipids and proteins from oxidative damage, and supports peroxisome–mitochondria metabolic coordination. Cat activity interfaces with antioxidant defense networks and inflammatory stress responses, influencing processes such as senescence, apoptosis regulation, and metabolic adaptation. Altered catalase function or expression is frequently studied in contexts of oxidative stress–associated phenotypes, including neurodegeneration, cardiometabolic dysfunction, and tissue injury models in mouse systems.

catalase CRISPR Activation Plasmid (m) provides a targeted, non-destructive approach to upregulating endogenous Cat expression without altering the underlying DNA sequence.

catalase CRISPR Activation Plasmid (m) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the Cat locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.

Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the Cat transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous catalase expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native Cat locus and enabling the study of catalase-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of catalase pathway restoration in tumor cells with silenced or reduced Cat expression.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.