caspase-3 Double Nickase Plasmid (m): sc-419466-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- caspase-3 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- caspase-3 Double Nickase Plasmid (m) and caspase-3 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Casp3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: caspase-3 p11 Antibody (C-6): sc-271759
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caspase-3 Double Nickase Plasmid (m) | sc-419466-NIC | 20 µg | $410.00 | |||
caspase-3 Double Nickase Plasmid (m2) | sc-419466-NIC-2 | 20 µg | $410.00 |
Casp3 encodes caspase-3, a central executioner protease in apoptosis that is activated downstream of intrinsic mitochondrial signaling and extrinsic death receptor pathways. Once processed by initiator caspases, caspase-3 cleaves numerous substrates to drive chromatin condensation, DNA fragmentation, membrane blebbing, and apoptotic body formation. In mouse models, Casp3 activity shapes tissue development and homeostasis and influences responses to cellular stress, immune-mediated cytotoxicity, and neurodegenerative or inflammatory processes where dysregulated cell death contributes to pathology. Because caspase-3 sits at a convergence point for pro-apoptotic signaling, perturbing Casp3 is widely used to dissect crosstalk between apoptosis, compensatory survival pathways, and regulated necrosis.
caspase-3 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Casp3 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Casp3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Casp3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Casp3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.