Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CALML3 CRISPR/Cas9 KO Plasmid (h): sc-400954
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CALML3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CALML3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CALML3 CRISPR/Cas9 KO Plasmid (h) | sc-400954 | 20 µg | $397.00 |
Overview
CALML3 encodes calmodulin-like 3, an EF-hand calcium-binding protein that functions as a calcium sensor modulating calmodulin-dependent signaling in a context-dependent manner. CALML3 is enriched in epithelial tissues and has been linked to regulation of cytoskeletal organization, membrane dynamics, and differentiation programs through calcium-driven control of kinase and phosphatase activities. By coupling intracellular Ca²⁺ flux to downstream effectors, CALML3 can influence processes such as cell adhesion, motility, and barrier-related remodeling. Altered CALML3 expression patterns have been reported in epithelial pathobiology and cancer-associated phenotypes, supporting its study in signaling rewiring and cellular state transitions.
CALML3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CALML3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CALML3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CALML3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CALML3 protein expression.
This CRISPR knockout system enables efficient generation of CALML3-deficient cell models for investigation of CALML3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CALML3 exon(s) critical for CALML3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CALML3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CALML3 CRISPR/Cas9 KO Plasmid (h) and CALML3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CALML3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CALML3 HDR Plasmid (h) and CALML3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CALML3 homology arms to support homology-directed repair at defined CALML3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.