C4α Antibody (C-2): sc-271181

C4α Antibody (C-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects the C4 alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). C4α (C-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The C4 alpha protein plays a crucial role in the complement system, which is essential for immune defense and inflammation regulation. C4 alpha is synthesized as a precursor that is cleaved into three chains: α, β, and γ, which are non-identical cleavage products. The α chain can further be cleaved into two isotypes, C4a and C4b, each with distinct reactivity; C4a interacts with amino groups, while C4b reacts with hydroxyl groups. This functional diversity is vital for the complement system′s ability to target pathogens and modulate immune responses. Notably, the human C4 gene is polymorphic at two loci, C4A and C4B, located on chromosome 6p21.3, with C4A expressing the Rodgers blood group antigen and C4B expressing the Chido blood group antigen. Deficiencies in the C4 gene have been linked to autoimmune disorders, such as systemic lupus erythematosus, highlighting anti-C4α antibody (C-2)′s importance in both research and clinical diagnostics.

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C4α Antibody (C-2) References:

1. Two clusters of acidic amino acids near the NH2 terminus of complement component C4 alpha'-chain are important for C2 binding.  |  Pan, Q., et al. 2000. J Immunol. 165: 2518-27. PMID: 10946278
2. Genetic, structural and functional diversities of human complement components C4A and C4B and their mouse homologues, Slp and C4.  |  Blanchong, CA., et al. 2001. Int Immunopharmacol. 1: 365-92. PMID: 11367523
3. Genetics of human complement component C4 and evolution the central MHC.  |  Martinez, OP., et al. 2001. Front Biosci. 6: D904-13. PMID: 11487475
4. Characterization of a de novo conversion in human complement C4 gene producing a C4B5-like protein.  |  Jaatinen, T., et al. 2002. J Immunol. 168: 5652-8. PMID: 12023363
5. The molecular basis of complete complement C4A and C4B deficiencies in a systemic lupus erythematosus patient with homozygous C4A and C4B mutant genes.  |  Rupert, KL., et al. 2002. J Immunol. 169: 1570-8. PMID: 12133986
6. Conversion of the C4d.2 serologic allotype of murine complement component C4 to the C4d.1 allotype by site-specific mutagenesis.  |  Mathias, P., et al. 1990. J Immunol. 144: 607-9. PMID: 2295802
7. Structural basis of the polymorphism of human complement components C4A and C4B: gene size, reactivity and antigenicity.  |  Yu, CY., et al. 1986. EMBO J. 5: 2873-81. PMID: 2431902
8. Studies on murine Ss protein: demonstration that S region encodes structural gene for fourth component of complement.  |  Carroll, MC. and Capra, JD. 1979. Proc Natl Acad Sci U S A. 76: 4641-5. PMID: 291992
9. Structure and function of the anaphylatoxins.  |  Hugli, TE. 1984. Springer Semin Immunopathol. 7: 193-219. PMID: 6387982
10. Post-translational modification of the fourth component of complement. Sulfation of the alpha-chain.  |  Karp, DR. 1983. J Biol Chem. 258: 12745-8. PMID: 6630204
11. Partial amino acid sequences of the murine fourth component of complement (C4): demonstration of homology with human C4 and identification of the amino-terminal subunit in pro-C4.  |  Parker, KL., et al. 1980. Proc Natl Acad Sci U S A. 77: 4275-8. PMID: 6933477
12. Molecular characterization of complement components (C3, C4, and factor B) in human saliva.  |  Andoh, A., et al. 1997. J Clin Immunol. 17: 404-7. PMID: 9327340

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-C4α Antibody (C-2) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-C4α Antibody (C-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

C4α Antibody (C-2) Specifications

Alternate Product Name: C4α

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1146-1300

Epitope Target: C4

Epitope Species: human

Product Reactivity/Detection: C4 mono precursor, C4 α chain and C4b

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for C4α Antibody (C-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin