C/EBP beta Double Nickase Plasmid (m): sc-419623-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C/EBP beta Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C/EBP beta Double Nickase Plasmid (m) and C/EBP beta Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cebpb. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: C/EBP beta Antibody (H-7): sc-7962
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C/EBP beta Double Nickase Plasmid (m) | sc-419623-NIC | 20 µg | $410.00 | |||
C/EBP beta Double Nickase Plasmid (m2) | sc-419623-NIC-2 | 20 µg | $410.00 |
Mouse Cebpb encodes C/EBP beta, a basic leucine zipper transcription factor that coordinates inducible gene expression programs in response to inflammatory cues, metabolic stress, and differentiation signals. C/EBP beta integrates inputs from cytokine and Toll-like receptor pathways and cooperates with factors such as NF-κB and STATs to regulate myeloid lineage commitment, acute-phase responses, and adipogenesis. Its activity influences cell-cycle control, survival, and tissue remodeling through transcriptional regulation of immune and metabolic gene networks. Dysregulated C/EBP beta signaling is frequently studied in contexts of chronic inflammation, metabolic dysfunction, and oncogenic transcriptional rewiring in mammalian models.
C/EBP beta Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cebpb locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cebpb. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cebpb function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cebpb-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.