C/EBP α CRISPR/Cas9 KO Plasmid (h): sc-400195

Overview

CEBPA encodes the transcription factor C/EBPα, a basic leucine zipper protein that binds CCAAT/enhancer elements to coordinate lineage-specific gene expression programs. It is a key regulator of hematopoietic and myeloid differentiation, integrating signaling with transcriptional control of cell-cycle arrest, metabolic homeostasis, and granulocytic maturation. C/EBPα interfaces with pathways governing chromatin remodeling and transcriptional networks involving cytokine signaling and other myeloid regulators, shaping innate immune development. Dysregulation of CEBPA expression or function is linked to altered differentiation states and proliferative phenotypes, making it widely studied in contexts such as hematologic malignancy biology and stem/progenitor cell fate decisions.

C/EBP α CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CEBPA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CEBPA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CEBPA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C/EBP α protein expression.

This CRISPR knockout system enables efficient generation of CEBPA-deficient cell models for investigation of C/EBP α signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting CEBPA exon(s) critical for C/EBP α function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple CEBPA genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by C/EBP α CRISPR/Cas9 KO Plasmid (h) and C/EBP α CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CEBPA locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by C/EBP α HDR Plasmid (h) and C/EBP α HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CEBPA homology arms to support homology-directed repair at defined CEBPA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.