Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BTBD2 CRISPR/Cas9 KO Plasmid (m): sc-431551
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BTBD2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BTBD2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BTBD2 Antibody (H-2): sc-393927
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BTBD2 CRISPR/Cas9 KO Plasmid (m) | sc-431551 | 20 µg | $397.00 |
Overview
Btbd2 encodes BTBD2, a BTB/POZ domain–containing protein thought to function as an adaptor in protein–protein interaction networks that influence ubiquitin-dependent proteostasis and regulated turnover of cellular factors. BTBD2-associated processes have been linked to control of signaling amplitude and stress-responsive pathways through modulation of protein stability, with downstream effects on cell-cycle progression and differentiation programs. In mouse systems, perturbation of Btbd2 provides a means to interrogate how BTB domain scaffolds contribute to tissue homeostasis and developmental phenotypes. Dysregulated proteostasis and aberrant signaling control are broadly relevant to disease biology, including contexts involving proliferative defects and neurodevelopmental vulnerability.
BTBD2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Btbd2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Btbd2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Btbd2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BTBD2 protein expression.
This CRISPR knockout system enables efficient generation of Btbd2-deficient cell models for investigation of BTBD2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Btbd2 exon(s) critical for BTBD2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Btbd2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BTBD2 CRISPR/Cas9 KO Plasmid (m) and BTBD2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Btbd2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BTBD2 HDR Plasmid (m) and BTBD2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Btbd2 homology arms to support homology-directed repair at defined Btbd2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.