Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BRD1 CRISPR/Cas9 KO Plasmid (h): sc-406252
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BRD1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BRD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BRD1 Antibody (F-12): sc-398226
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRD1 CRISPR/Cas9 KO Plasmid (h) | sc-406252 | 20 µg | $397.00 |
Overview
Human BRD1 (bromodomain containing 1) is a chromatin-associated regulator that recognizes acetylated lysine residues on histone tails via its bromodomain, linking histone acetylation to transcriptional control. BRD1 participates in epigenetic programs that shape gene expression during development and cellular differentiation, including regulation of promoter and enhancer activity through chromatin remodeling and co-regulator recruitment. By modulating transcriptional networks connected to neuronal and immune-related pathways, BRD1 has been studied in the context of altered gene expression states relevant to neuropsychiatric and other complex, polygenic disorders. Its role at the interface of histone acetylation and transcription makes BRD1 a useful target for dissecting chromatin-dependent mechanisms that influence cell identity and stress responses.
BRD1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BRD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BRD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BRD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BRD1 protein expression.
This CRISPR knockout system enables efficient generation of BRD1-deficient cell models for investigation of BRD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BRD1 exon(s) critical for BRD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BRD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BRD1 CRISPR/Cas9 KO Plasmid (h) and BRD1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BRD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BRD1 HDR Plasmid (h) and BRD1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BRD1 homology arms to support homology-directed repair at defined BRD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.