BLM Double Nickase Plasmid (m): sc-419336-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BLM Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BLM Double Nickase Plasmid (m) and BLM Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Blm. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BLM Antibody (B-4): sc-365753
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BLM Double Nickase Plasmid (m) | sc-419336-NIC | 20 µg | $410.00 | |||
BLM Double Nickase Plasmid (m2) | sc-419336-NIC-2 | 20 µg | $410.00 |
Mouse Blm encodes the BLM RecQ-family DNA helicase, a key genome maintenance factor that resolves aberrant DNA secondary structures and promotes accurate homologous recombination. BLM functions in replication fork restart, end resection, and dissolution of double Holliday junctions in concert with the BTR complex (BLM–TOP3A–RMI1/2), thereby limiting crossover events and chromosomal rearrangements. Through these activities, BLM interfaces with DNA damage response pathways involving ATR/CHK1 signaling and the Fanconi anemia network during replication stress. Disruption of BLM function is associated with elevated sister chromatid exchange, genome instability, and cancer-predisposition phenotypes, making Blm a useful locus for studying DNA repair defects and mutational processes in mammalian cells.
BLM Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Blm locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Blm. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Blm function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Blm-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.