Anticorpo beta-defensin 2 (2-RY8) raccomandato per il rilevamento di β-defensin 2 di origine human in WB, IP e ELISA

Anticorpo β-defensin 2 (2-RY8): sc-134314

Anticorpo β-defensin 2 (2-RY8) valutazione 3.5 di 5 di 4.
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5
2
4
0
3
1
2
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Schede tecniche

    • Anti-beta-defensin 2 Antibody 2-RY8 è un monoclonale di topo IgG1 (kappa light chain) β-defensin 2 antibody fornito in 100 µg/ml
    • raised against a partial recombinant fragment of β-defensin 2 of human origin
    • raccomandato per il rilevamento di β-defensin 2 di origine human in WB, IP e ELISA
    • Contattaci per ricevere un FREE 10 µg sample di β-defensin 2 (2-RY8): sc-134314.

For Western Blot, is it recommended to use denatured or non-denatured conditions with β-defensin 2 (2-RY8): sc-134314 antibody?

Richiesta da: AbPolly
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Risposta da: Technical Support
Data di pubblicazione: 2017-03-27
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Rated 1 di 5 di da IHC Assay Review for Beta-Defensin-2 mAB Application: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: Specific stain not detected even at dilution factor of 10.
Data di pubblicazione: 2017-08-31
Rated 5 di 5 di da western blot detection It worked with succes for western blot detect β-defensin 2,WB 1:1000,The samples were 293T cells,the band at 5kda.Recommend use 15% SDS-PAGE,since the MW is low.
Data di pubblicazione: 2017-08-12
Rated 5 di 5 di da Western blot Western blot detection of good human cells, WB diluted 1:1000 clear band.
Data di pubblicazione: 2017-05-24
Rated 3 di 5 di da Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Data di pubblicazione: 2013-04-19
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