BCoR Double Nickase Plasmid (m): sc-427958-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BCoR Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BCoR Double Nickase Plasmid (m) and BCoR Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Bcor. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BCoR Double Nickase Plasmid (m) | sc-427958-NIC | 20 µg | $410.00 |
Bcor encodes BCoR, a BCL6-interacting transcriptional corepressor that modulates gene expression programs by coordinating epigenetic repression. In mouse cells, BCoR functions within chromatin-associated complexes that interface with Polycomb group regulation and histone modification pathways to control lineage specification, differentiation, and cell-cycle–linked transcriptional states. Through these mechanisms, BCoR influences developmental and immune-related transcriptional networks and has been studied in the context of dysregulated transcriptional repression. Altered BCOR activity is associated with defects in developmental patterning and hematopoietic gene regulation, making Bcor a useful locus for investigating epigenetic control of cell fate.
BCoR Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Bcor locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Bcor. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Bcor function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Bcor-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.