Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BACE Double Nickase Plasmid (m): sc-423847-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BACE Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BACE Double Nickase Plasmid (m) and BACE Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Bace1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BACE Antibody (61-3E7): sc-33711
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BACE Double Nickase Plasmid (m) | sc-423847-NIC | 20 µg | $410.00 | |||
BACE Double Nickase Plasmid (m2) | sc-423847-NIC-2 | 20 µg | $410.00 |
Mouse Bace1 encodes β-site amyloid precursor protein cleaving enzyme 1 (BACE), an aspartyl protease that initiates amyloidogenic processing of APP by generating the C99 fragment that is subsequently cleaved by γ-secretase. BACE activity intersects with endosomal trafficking and regulated intramembrane proteolysis, and it contributes to proteostasis and membrane protein turnover in neurons and other cell types. Altered Bace1 expression or activity has been linked to amyloid-β production and synaptic dysfunction, making it a widely used target for mechanistic studies of neurodegeneration-relevant pathways. Experimental modulation of Bace1 supports investigation of APP processing dynamics, endolysosomal function, and downstream signaling changes in cellular and mouse model systems.
BACE Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Bace1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Bace1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Bace1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Bace1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.