BABAM1 Double Nickase Plasmid (h): sc-409906-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BABAM1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BABAM1 Double Nickase Plasmid (h) and BABAM1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BABAM1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BABAM1 Antibody (H-10): sc-398570
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BABAM1 Double Nickase Plasmid (h) | sc-409906-NIC | 20 µg | $410.00 | |||
BABAM1 Double Nickase Plasmid (h2) | sc-409906-NIC-2 | 20 µg | $410.00 |
BABAM1 (also known as MERIT40) encodes a core component of the BRCA1-A complex that targets BRCA1 to DNA double-strand breaks and helps coordinate ubiquitin-dependent signaling at sites of genotoxic stress. By binding partners such as RAP80, ABRAXAS1, and BRE/BRCC45, BABAM1 supports assembly of a deubiquitinating module that modulates K63-linked ubiquitin chains and regulates DNA damage checkpoint control and homologous recombination pathway choice. This activity links BABAM1 to maintenance of genome stability, replication stress responses, and cell cycle progression. Dysregulation of BRCA1-A complex components, including BABAM1, has been associated with altered DNA repair capacity and genomic instability phenotypes relevant to cancer biology and other disorders of DNA damage response.
BABAM1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BABAM1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BABAM1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BABAM1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BABAM1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.