AZ1 CRISPR/Cas9 KO Plasmid (h): sc-406801
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AZ1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AZ1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AZ1 CRISPR/Cas9 KO Plasmid (h) | sc-406801 | 20 µg | $397.00 |
Overview
Human OAZ1 encodes antizyme 1 (AZ1), a key negative regulator of polyamine metabolism that limits intracellular putrescine, spermidine, and spermine by binding ornithine decarboxylase (ODC) and promoting its ubiquitin-independent proteasomal degradation. By constraining polyamine biosynthesis and transport, AZ1 influences core processes including cell-cycle progression, translation, and stress-adaptive growth programs. OAZ1 expression is tightly controlled by a polyamine-responsive +1 ribosomal frameshift, forming a feedback circuit that stabilizes polyamine homeostasis. Dysregulated polyamine control involving OAZ1 has been associated with proliferative signaling states and altered metabolic dependencies observed in cancer and other growth-related pathologies.
AZ1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OAZ1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OAZ1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OAZ1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AZ1 protein expression.
This CRISPR knockout system enables efficient generation of OAZ1-deficient cell models for investigation of AZ1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OAZ1 exon(s) critical for AZ1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OAZ1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AZ1 CRISPR/Cas9 KO Plasmid (h) and AZ1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OAZ1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AZ1 HDR Plasmid (h) and AZ1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OAZ1 homology arms to support homology-directed repair at defined OAZ1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.