ASIC1 Double Nickase Plasmid (h): sc-401452-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ASIC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ASIC1 Double Nickase Plasmid (h) and ASIC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ASIC1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASIC1 Double Nickase Plasmid (h) | sc-401452-NIC | 20 µg | $410.00 | |||
ASIC1 Double Nickase Plasmid (h2) | sc-401452-NIC-2 | 20 µg | $410.00 |
Human ASIC1 (acid-sensing ion channel 1, ASIC1) encodes a proton-gated, sodium-permeable ion channel of the DEG/ENaC family that couples extracellular acidification to rapid membrane depolarization. ASIC1 activity shapes neuronal excitability and synaptic signaling and can influence intracellular calcium dynamics through secondary activation of voltage-gated channels and downstream kinase signaling. The channel participates in pH-sensing processes linked to ischemia-associated acidosis, nociceptive transmission, and neuroinflammatory microenvironments. Dysregulated ASIC1 function or expression has been investigated in contexts including stroke susceptibility, chronic pain states, and anxiety-related behaviors, supporting its utility as a mechanistic target in neurobiology and ion channel research.
ASIC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ASIC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ASIC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ASIC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ASIC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.