Aquaporin 8/AQP8 Double Nickase Plasmid (h): sc-402490-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 8/AQP8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Aquaporin 8/AQP8 Double Nickase Plasmid (h) and Aquaporin 8/AQP8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AQP8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 8/AQP8 Antibody (14-Z): sc-81870
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 8/AQP8 Double Nickase Plasmid (h) | sc-402490-NIC | 20 µg | $410.00 | |||
Aquaporin 8/AQP8 Double Nickase Plasmid (h2) | sc-402490-NIC-2 | 20 µg | $410.00 |
AQP8 encodes aquaporin 8, an integral membrane channel that facilitates transmembrane water transport and can also conduct small neutral solutes such as hydrogen peroxide depending on cellular context. AQP8 contributes to osmotic homeostasis, epithelial fluid handling, and intracellular redox signaling, linking membrane permeability to processes including mitochondrial function, metabolic regulation, and inflammatory signaling. Altered AQP8 expression or localization has been associated with epithelial barrier dysfunction and dysregulated oxidative stress responses, features observed across multiple pathophysiological settings. In human tissues, AQP8 is frequently studied in relation to hepatobiliary physiology, gastrointestinal epithelial transport, and immune-cell oxidative burst modulation.
Aquaporin 8/AQP8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AQP8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AQP8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AQP8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AQP8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.