Anticorpo apoE (F-9) raccomandato per il rilevamento di apoE di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo apoE (F-9): sc-390925

Anticorpo apoE (F-9) valutazione 4.7 di 5 di 10.
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Schede tecniche

    • apoE Antibody F-9 è un monoclonale di topo IgG2a fornito in 200 µg/ml
    • generato contro gli amminoacidi 31-253 mappati nella regione interna di apoE di origine human
    • apoE Antibody (F-9) é raccomandato per il rilevamento di apoE di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • apoE Antibody (F-9) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Vedi m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), le nostre alternative ricombinanti ai reagenti secondari convenzionali anti-mouse IgG altamente raccomandate.
    • Contattaci per ricevere un FREE 10 µg sample di apoE (F-9): sc-390925.

apoE F-9 has replaced M-20. However, M-20 binds on the C-terminus of apoE, while F-9 binds within an internal region of apoE. Therefore, by F-9 we cannot detect apoE fragments previously detected with M-20. Any suggestion for another replacement?

Richiesta da: Aggelika
Thank you for your question. You can find all antibodies we offer against apoE by following this link: https://www.scbt.com/browse/apoe-Antik%C3%B6rper/_/N-pr9qxd?&_requestid=4836 apoE Antibody (A1.4): sc-13521, for instance, is specific against amino acids 126-191 of apoE of human origin.
Risposta da: Tech Support Europe
Data di pubblicazione: 2019-11-22

Does this APOE antibody (F-9) have different affinity to different APOE isoforms (E2, E3, and E4)?

Richiesta da: CSYang
Thank you for your question. We have not performed any testing with this apoE Antibody (F-9): sc-390925 to determine reactivity with, or affinity for the different apoE isoforms, E2, E3 and E4.
Risposta da: Technical Support
Data di pubblicazione: 2017-05-17

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Richiesta da: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Risposta da: Technical Support 14
Data di pubblicazione: 2017-01-26

The epitope mapping does not make sense - this protein is 319 aa long if including the signaling peptide. Please could you clarify this epitope sequence?

Richiesta da: Jade
Thank you for your question, and for bringing this discrepancy to our attention. After reviewing the product, we have made a typo in the amino acid range listed as the immunogen for this antibody and incorrectly listed the range as 31-353. The correct range is 31-253. This antibody, apoE Antibody (F-9): sc-390925, is a mouse monoclonal antibody raised against a recombinant protein corresponding to amino acids 31-253 of apoE of human origin.
Risposta da: Technical Support
Data di pubblicazione: 2017-03-02
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Rated 5 di 5 di da Research associate It works very well in western blotting, strong signal and low back grand..
Data di pubblicazione: 2018-11-29
Rated 5 di 5 di da works for both mouse and human APOEs We tried this APOE ab as a sample. The bands were pretty clear and no band in our KO mouse Brian samples or human ApoE KO lines.
Data di pubblicazione: 2018-04-05
Rated 5 di 5 di da dilution 1:200 Antibody can detect apoE by WB, the band is clear, antibody is very good
Data di pubblicazione: 2017-09-07
Rated 5 di 5 di da very good antibody Clean WB blot with single strong band in human liver tissue extract and 293T whole cell lysate.
Data di pubblicazione: 2017-08-11
Rated 3 di 5 di da apoE WB:there is a clear bands in mouse liver tissue,and used 1:500 dilution
Data di pubblicazione: 2017-08-09
Rated 4 di 5 di da Works well for IF in fetal RPE cells Good signal in differentiated fetal RPE cells by immunofluorescence.
Data di pubblicazione: 2017-04-20
Rated 5 di 5 di da Strong signal Very strong and specific signal using apoE Antibody (F-9) by WB and IHCP
Data di pubblicazione: 2017-01-24
Rated 5 di 5 di da Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Data di pubblicazione: 2015-05-03
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