AP-2β Antibody (1F3/8): sc-53160

AP-2β Antibody (1F3/8) is a mouse monoclonal IgG2a antibody that detects AP-2β in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). AP-2β, a crucial member of the AP-2 transcription factor family, plays a significant role in gene regulation by binding to the DNA consensus sequence CCCCAGGC, thereby initiating transcription of specific target genes. AP-2β is a splice variant of AP-2α and serves as a negative regulator of AP-2 activity, which is essential for maintaining the balance of gene expression in various biological processes. AP-2β is primarily located in the nucleus, where AP-2β influences the transcriptional regulation of genes involved in cell growth and differentiation. This localization allows AP-2β to interact with other transcription factors and co-regulators, thereby modulating the expression of genes linked to critical cellular functions, including those associated with cancer progression. AP-2β is involved in the development of ectodermal-derived tissues, and mutations in AP-2β can lead to developmental disorders, highlighting AP-2β′s importance in both normal physiology and disease states. Through interaction with the OB2-1 transcription factor complex, AP-2β also plays a role in the upregulation of the proto-oncogene c-ErbB-2, which is overexpressed in a significant percentage of breast cancers, making AP-2β a key player in oncogenic signaling pathways.

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AP-2β Antibody (1F3/8) References:

1. Differential expression of AP-2alpha and AP-2beta in the developing chick retina: repression of R-FABP promoter activity by AP-2.  |  Bisgrove, DA. and Godbout, R. 1999. Dev Dyn. 214: 195-206. PMID: 10090146
2. AP-2β is a transcriptional regulator for determination of digit length in tetrapods.  |  Seki, R., et al. 2015. Dev Biol. 407: 75-89. PMID: 26277217
3. AP-2α and AP-2β regulate dorsal interneuron specification in the spinal cord.  |  Xu, X., et al. 2017. Neuroscience. 340: 232-242. PMID: 27984181
4. Transcription factor AP-2beta in development, differentiation and tumorigenesis.  |  Raap, M., et al. 2021. Int J Cancer. 149: 1221-1227. PMID: 33720400
5. AP-2α and AP-2β cooperatively function in the craniofacial surface ectoderm to regulate chromatin and gene expression dynamics during facial development.  |  Van Otterloo, E., et al. 2022. Elife. 11: PMID: 35333176
6. Transcription factors AP-2α and AP-2β regulate distinct segments of the distal nephron in the mammalian kidney.  |  Lamontagne, JO., et al. 2022. Nat Commun. 13: 2226. PMID: 35468900
7. The regulatory role of AP-2β in monoaminergic neurotransmitter systems: insights on its signalling pathway, linked disorders and theragnostic potential.  |  Al-Sabri, MH., et al. 2022. Cell Biosci. 12: 151. PMID: 36076256
8. Ap-2β regulates cranial osteogenic potential via the activation of Wnt/β-catenin signaling pathway.  |  Hu, S., et al. 2023. Dev Biol. 501: 81-91. PMID: 37355029
9. AP-2α/AP-2β Transcription Factors Are Key Regulators of Epidermal Homeostasis.  |  Zhang, H., et al. 2024. J Invest Dermatol. 144: 1505-1521.e12. PMID: 38237728
10. Autophagy initiation triggers p150Glued-AP-2β interaction on the lysosomes and facilitates their transport.  |  Tempes, A., et al. 2024. Cell Mol Life Sci. 81: 218. PMID: 38758395

The preferred AP-2β antibody is AP-2β Antibody (C-6): sc-390119.