AP-2α/β Antibody (3H547): sc-70361

AP-2α/β Antibody (3H547) is a mouse monoclonal IgG1 antibody that detects AP-2α/β in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). AP-2α/β is a crucial member of the AP-2 transcription factor family, which plays a significant role in gene regulation by binding to the DNA consensus sequence CCCCAGGC, thereby initiating transcription of specific target genes. AP-2α is involved in the regulation of estrogen receptor expression, which is vital for normal cellular function and development, particularly in ectodermal-derived tissues. AP-2α′s importance is underscored by its association with various developmental disorders; deleterious mutations in AP-2α have been linked to conditions such as microphthalmia and corneal clouding, highlighting AP-2α′s critical role in eye development. AP-2α interacts with other members of the AP-2 family, including AP-2β, which can inhibit AP-2 activity, and together they form the OB2-1 transcription factor complex that upregulates the proto-oncogene c-ErbB-2, often overexpressed in breast cancer. Anti-AP-2α/β antibody (3H547)′s versatility in multiple applications makes this reagent an essential tool for researchers studying gene regulation and its implications in various diseases.

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AP-2α/β Antibody (3H547) References:

1. AP-2α and AP-2β regulate dorsal interneuron specification in the spinal cord.  |  Xu, X., et al. 2017. Neuroscience. 340: 232-242. PMID: 27984181
2. AP-2α and AP-2β cooperatively orchestrate homeobox gene expression during branchial arch patterning.  |  Van Otterloo, E., et al. 2018. Development. 145: PMID: 29229773
3. The biological characteristics of transcription factors AP-2α and AP-2γ and their importance in various types of cancers.  |  Kołat, D., et al. 2019. Biosci Rep. 39: PMID: 30824562
4. Transcription factor AP-2beta in development, differentiation and tumorigenesis.  |  Raap, M., et al. 2021. Int J Cancer. 149: 1221-1227. PMID: 33720400
5. A positive feedback loop between Periostin and TGFβ1 induces and maintains the stemness of hepatocellular carcinoma cells via AP-2α activation.  |  Chen, G., et al. 2021. J Exp Clin Cancer Res. 40: 218. PMID: 34193219
6. AP-2α-Mediated Activation of E2F and EZH2 Drives Melanoma Metastasis.  |  White, JR., et al. 2021. Cancer Res. 81: 4455-4470. PMID: 34210752
7. Prognostic significance of AP-2α/γ targets as cancer therapeutics.  |  Kołat, D., et al. 2022. Sci Rep. 12: 5497. PMID: 35361846
8. Transcription factors AP-2α and AP-2β regulate distinct segments of the distal nephron in the mammalian kidney.  |  Lamontagne, JO., et al. 2022. Nat Commun. 13: 2226. PMID: 35468900
9. Epigenetically modified AP-2α by DNA methyltransferase facilitates glioma immune evasion by upregulating PD-L1 expression.  |  Long, S., et al. 2023. Cell Death Dis. 14: 365. PMID: 37330579
10. AP-2α/AP-2β Transcription Factors Are Key Regulators of Epidermal Homeostasis.  |  Zhang, H., et al. 2024. J Invest Dermatol. 144: 1505-1521.e12. PMID: 38237728

The preferred AP-2β antibody is AP-2β Antibody (C-6): sc-390119.