ANP32B Double Nickase Plasmid (m): sc-426661-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANP32B Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ANP32B Double Nickase Plasmid (m) and ANP32B Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Anp32b. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANP32B Double Nickase Plasmid (m) | sc-426661-NIC | 20 µg | $410.00 | |||
ANP32B Double Nickase Plasmid (m2) | sc-426661-NIC-2 | 20 µg | $410.00 |
Anp32b encodes acidic nuclear phosphoprotein 32 family member B (ANP32B), a conserved nuclear protein that participates in chromatin-associated regulation of transcription, RNA processing, and cell cycle control. ANP32B has been linked to modulation of histone acetylation dynamics and protein phosphatase signaling, supporting programs that influence proliferation, differentiation, and cellular stress responses. In mouse systems, perturbation of Anp32b can impact apoptosis susceptibility and inflammatory or antiviral transcriptional outputs, making it relevant to studies of tumor biology, neurobiology, and host–pathogen interactions. Its nuclear localization and scaffolding-like features also position ANP32B as a useful node for dissecting epigenetic regulation and RNA metabolism pathways.
ANP32B Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Anp32b locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Anp32b. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Anp32b function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Anp32b-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.