Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ANKTM1 Double Nickase Plasmid (m): sc-435491-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANKTM1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ANKTM1 Double Nickase Plasmid (m) and ANKTM1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Trpa1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANKTM1 Double Nickase Plasmid (m) | sc-435491-NIC | 20 µg | $410.00 |
Mouse Trpa1 encodes ANKTM1 (TRPA1), a polymodal, nonselective cation channel of the transient receptor potential family that integrates electrophilic irritants, reactive oxygen/nitrogen species, and inflammatory mediators to regulate sensory neuron excitability. Channel activation drives calcium influx and downstream signaling that shapes nociception, neurogenic inflammation, and airway and vascular responses, linking TRPA1 to processes such as oxidative stress sensing and GPCR- and kinase-modulated ion channel gating. TRPA1 activity intersects with inflammatory pathways through mediator release and neuron–immune crosstalk, influencing pain hypersensitivity and itch phenotypes in preclinical models. Dysregulated TRPA1 signaling has been associated with inflammatory pain states, neuropathic pain mechanisms, cough and asthma-like airway hyperreactivity, and migraine-related sensory processing, supporting its broad relevance in neurobiology and immunophysiology research.
ANKTM1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Trpa1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Trpa1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Trpa1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Trpa1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.