Amylase 1 CRISPR/Cas9 KO Plasmid (m): sc-419103
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Amylase 1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Amylase 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Amylase Antibody (C-12): sc-514313
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Amylase 1 CRISPR/Cas9 KO Plasmid (m) | sc-419103 | 20 µg | $397.00 |
Overview
Mouse Amy1 encodes amylase 1, a secreted glycoside hydrolase that catalyzes endohydrolysis of α-1,4-glycosidic bonds in starch and glycogen to generate maltose and oligosaccharides. By governing the earliest steps of carbohydrate digestion, AMY1 influences luminal glucose availability and downstream metabolic signaling, including nutrient-sensing pathways that shape epithelial and microbial ecology. Variation in amylase activity has been linked to dietary carbohydrate handling and has been explored in the context of metabolic phenotypes such as obesity and insulin resistance, supporting its relevance to nutrition–metabolism interactions. In mice, Amy1 provides a tractable model for studying digestive enzyme regulation, secretory protein biology, and gene–diet effects on systemic physiology.
Amylase 1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Amy1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Amy1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Amy1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Amylase 1 protein expression.
This CRISPR knockout system enables efficient generation of Amy1-deficient cell models for investigation of Amylase 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Amy1 exon(s) critical for Amylase 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Amy1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Amylase 1 CRISPR/Cas9 KO Plasmid (m) and Amylase 1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Amy1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Amylase 1 HDR Plasmid (m) and Amylase 1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Amy1 homology arms to support homology-directed repair at defined Amy1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.