AMDHD2 CRISPR/Cas9 KO Plasmid (m): sc-434174
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AMDHD2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AMDHD2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AMDHD2 CRISPR/Cas9 KO Plasmid (m) | sc-434174 | 20 µg | $397.00 |
Overview
Amdhd2 encodes amidohydrolase domain containing 2 (AMDHD2), a cytosolic metallo-dependent deacetylase that participates in amino-sugar metabolism by catalyzing deacetylation steps linked to N-acetylglucosamine (GlcNAc) utilization. Through its role in processing glucosamine-derived intermediates, AMDHD2 can influence cellular nutrient sensing and pathways connected to hexosamine flux, which intersects with glycosylation capacity and metabolic homeostasis. Altered regulation of amino-sugar metabolism has been associated with changes in proliferation, stress responses, and differentiation programs in mammalian cells. In mouse models, perturbation of Amdhd2 provides a genetic entry point to study how GlcNAc-related metabolism contributes to tissue physiology and disease-relevant metabolic phenotypes.
AMDHD2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Amdhd2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Amdhd2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Amdhd2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AMDHD2 protein expression.
This CRISPR knockout system enables efficient generation of Amdhd2-deficient cell models for investigation of AMDHD2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Amdhd2 exon(s) critical for AMDHD2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Amdhd2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AMDHD2 CRISPR/Cas9 KO Plasmid (m) and AMDHD2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Amdhd2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AMDHD2 HDR Plasmid (m) and AMDHD2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Amdhd2 homology arms to support homology-directed repair at defined Amdhd2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.