Anticorpo alpha 1A-AR (4D8) raccomandato per il rilevamento di α1A-AR di origine mouse, rat e human in WB, IP e ELISA

Anticorpo α1A-AR (4D8): sc-100291

Anticorpo α1A-AR (4D8) valutazione 4.7 di 5 di 3.
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Schede tecniche

    • Anti-alpha 1A-AR Antibody 4D8 è un monoclonale di topo IgG1 (kappa light chain) α1A-AR antibody fornito in 100 µg/ml
    • raised against an N-terminal region of α1A-AR of human origin
    • raccomandato per il rilevamento di α1A-AR di origine mouse, rat e human in WB, IP e ELISA
    • Vedi m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), le nostre alternative ricombinanti ai reagenti secondari convenzionali anti-mouse IgG altamente raccomandate.
    • Contattaci per ricevere un FREE 10 µg sample di α1A-AR (4D8): sc-100291.

How selective is this antibody for alpha-1A? We need to address the selectivity issue in light of Michel et al. (2009) "How reliable are G-protein-coupled receptor antibodies?" NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY 379: 386-388.

Richiesta da: cpoon
Thank you for your question. The manuscript you mention recommends validating by IHC, but I would like to aslso suggest that also using siRNAs and CRISPR followed by a WB would also provide validation results to determine the reliability of the antibody. You can find recommended gene silencing products on the antibody product block: https://www.scbt.com/scbt/product/alpha1a-ar-antibody-4d8?requestFrom=search If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Risposta da: Tech Service
Data di pubblicazione: 2017-06-28

For Western Blot, is it recommended to use denatured or non-denatured conditions with α1A-AR (4D8): sc-100291 antibody?

Richiesta da: Germaine
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Risposta da: Technical Support
Data di pubblicazione: 2017-03-27
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Rated 5 di 5 di da Worked very well I used this antibody on mice tissues (liver, muscle, aorta) and it worked very well.
Data di pubblicazione: 2018-04-06
Rated 5 di 5 di da positive results Product sc-100291 is used for kinase assay using human myeloid cells,positive results!
Data di pubblicazione: 2016-12-17
Rated 4 di 5 di da Distinct band observed by WB using HL Distinct band observed by WB using HL-60 whole cell lysate. -SCBT QC
Data di pubblicazione: 2014-02-27
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