AIF Double Nickase Plasmid (m): sc-424143-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AIF Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AIF Double Nickase Plasmid (m) and AIF Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Aifm1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AIF Antibody (E-1): sc-13116
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AIF Double Nickase Plasmid (m) | sc-424143-NIC | 20 µg | $410.00 | |||
AIF Double Nickase Plasmid (m2) | sc-424143-NIC-2 | 20 µg | $410.00 |
Mouse Aifm1 encodes apoptosis-inducing factor (AIF), a flavoprotein localized primarily to the mitochondrial intermembrane space that supports oxidative phosphorylation and redox homeostasis. Upon severe cellular stress, AIF can translocate to the nucleus and promote caspase-independent chromatin condensation and large-scale DNA fragmentation, linking mitochondrial dysfunction to regulated cell death programs. Through these roles, AIF interfaces with mitochondrial quality control, reactive oxygen species signaling, and bioenergetic pathways that shape neuronal survival, muscle integrity, and immune cell homeostasis. Dysregulation of AIF-associated processes is relevant to studies of neurodegeneration, mitochondrial encephalomyopathies, ischemic injury models, and inflammatory pathophysiology in mice.
AIF Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Aifm1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Aifm1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Aifm1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Aifm1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.