AHCYL1 Antibody (H-1): sc-514560
- AHCYL1 Antibody (H-1) is a mouse monoclonal IgG2b κ AHCYL1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 46-68 near the N-terminus of AHCYL1 of human origin
- AHCYL1 Antibody (H-1) is recommended for detection of AHCYL1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-AHCYL1 Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for AHCYL1 Antibody (H-1) for WB applications. These reagents are now offered in bundles with AHCYL1 Antibody (H-1) (see ordering information below).
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AHCYL1 Antibody (H-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects AHCYL1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-AHCYL1 antibody (H-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AHCYL1, also known as S-adenosylhomocysteine hydrolase-like 1, DCAL, IRBIT, or PRO0233, is primarily located in the endoplasmic reticulum (ER) and plays a crucial role in amino acid biosynthesis. AHCYL1 catalyzes the hydrolytic conversion of S-adenosyl-L-homocysteine to L-homocysteine and adenosine, utilizing NAD as a cofactor in this essential metabolic pathway. This enzymatic activity is vital for maintaining cellular homocysteine levels, which are important for various physiological processes, including methylation reactions and neurotransmitter synthesis. AHCYL1 also features a PDZ-binding domain and a PEST region, allowing interaction with inositol 1,4,5-trisphosphate receptor-I (IP3R-I), a key player in intracellular signaling pathways. This interaction modulates IP3R-I affinity for its substrate, thereby influencing calcium signaling and cellular responses. AHCYL1 exhibits 100% homology with its mouse counterpart, underscoring conserved function across species, and two AHCYL1 isoforms arise from alternative splicing, adding to the complexity of regulatory roles in cellular metabolism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AHCYL1 Antibody (H-1) References:
- Identification of an S-adenosylhomocysteine hydrolase-like transcript induced during dendritic cell differentiation. | Dekker, JW., et al. 2002. Immunogenetics. 53: 993-1001. PMID: 11904675
- IRBIT, a novel inositol 1,4,5-trisphosphate (IP3) receptor-binding protein, is released from the IP3 receptor upon IP3 binding to the receptor. | Ando, H., et al. 2003. J Biol Chem. 278: 10602-12. PMID: 12525476
- Suppression and overexpression of adenosylhomocysteine hydrolase-like protein 1 (AHCYL1) influences zebrafish embryo development: a possible role for AHCYL1 in inositol phospholipid signaling. | Cooper, BJ., et al. 2006. J Biol Chem. 281: 22471-84. PMID: 16754674
- IRBIT, an inositol 1,4,5-trisphosphate receptor-binding protein, specifically binds to and activates pancreas-type Na+/HCO3- cotransporter 1 (pNBC1). | Shirakabe, K., et al. 2006. Proc Natl Acad Sci U S A. 103: 9542-7. PMID: 16769890
- IRBIT suppresses IP3 receptor activity by competing with IP3 for the common binding site on the IP3 receptor. | Ando, H., et al. 2006. Mol Cell. 22: 795-806. PMID: 16793548
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AHCYL1 Antibody (H-1) | sc-514560 | 200 µg/ml | $322.00 | |||
AHCYL1 Antibody (H-1): m-IgG Fc BP-HRP Bundle | sc-526378 | 200 µg Ab; 10 µg BP | $361.00 | |||
AHCYL1 Antibody (H-1): m-IgG2b BP-HRP Bundle | sc-549719 | 200 µg Ab; 10 µg BP | $361.00 | |||
AHCYL1 Antibody (H-1) AC | sc-514560 AC | 500 µg/ml, 25% agarose | $424.00 | |||
AHCYL1 Antibody (H-1) HRP | sc-514560 HRP | 200 µg/ml | $322.00 | |||
AHCYL1 Antibody (H-1) FITC | sc-514560 FITC | 200 µg/ml | $336.00 | |||
AHCYL1 Antibody (H-1) PE | sc-514560 PE | 200 µg/ml | $349.00 | |||
AHCYL1 Antibody (H-1) Alexa Fluor® 488 | sc-514560 AF488 | 200 µg/ml | $364.00 | |||
AHCYL1 Antibody (H-1) Alexa Fluor® 546 | sc-514560 AF546 | 200 µg/ml | $364.00 | |||
AHCYL1 Antibody (H-1) Alexa Fluor® 594 | sc-514560 AF594 | 200 µg/ml | $364.00 | |||
AHCYL1 Antibody (H-1) Alexa Fluor® 647 | sc-514560 AF647 | 200 µg/ml | $364.00 | |||
AHCYL1 Antibody (H-1) Alexa Fluor® 680 | sc-514560 AF680 | 200 µg/ml | $364.00 | |||
AHCYL1 Antibody (H-1) Alexa Fluor® 790 | sc-514560 AF790 | 200 µg/ml | $364.00 | |||
AHCYL1 (H-1) Neutralizing Peptide | sc-514560 P | 100 µg/0.5 ml | $69.00 |