Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ADPN Double Nickase Plasmid (h): sc-406096-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADPN Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ADPN Double Nickase Plasmid (h) and ADPN Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PNPLA3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ADPN Antibody (D-5): sc-390251
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADPN Double Nickase Plasmid (h) | sc-406096-NIC | 20 µg | $410.00 | |||
ADPN Double Nickase Plasmid (h2) | sc-406096-NIC-2 | 20 µg | $410.00 |
PNPLA3 encodes adiponutrin (ADPN), a patatin-like phospholipase localized to lipid droplets that regulates triglyceride hydrolysis and acyltransferase activity in hepatocytes and adipocytes. ADPN integrates nutrient and hormonal cues to modulate lipid remodeling, de novo lipogenesis coupling, and lipid droplet turnover, linking metabolic state to intracellular lipid storage. Genetic variation and altered PNPLA3 expression are strongly associated with hepatic fat accumulation and progression of fatty liver phenotypes, and are studied in the context of steatosis, inflammation, and fibrogenic signaling. As a result, PNPLA3/ADPN is widely used as a molecular entry point to interrogate lipid metabolism pathways and their downstream stress responses in human cell models.
ADPN Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PNPLA3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PNPLA3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PNPLA3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PNPLA3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.