ACSS1 Double Nickase Plasmid (h): sc-403211-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACSS1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ACSS1 Double Nickase Plasmid (h) and ACSS1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ACSS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACSS1 Antibody (D-7): sc-377149
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACSS1 Double Nickase Plasmid (h) | sc-403211-NIC | 20 µg | $410.00 | |||
ACSS1 Double Nickase Plasmid (h2) | sc-403211-NIC-2 | 20 µg | $410.00 |
ACSS1 encodes a mitochondrial acyl-CoA synthetase that catalyzes conversion of acetate to acetyl-CoA, linking acetate utilization to mitochondrial energy metabolism and anaplerotic flux through the TCA cycle. By supplying acetyl-CoA for oxidative metabolism and supporting lipid and ketone body-associated pathways, ACSS1 contributes to metabolic flexibility during nutrient stress and altered substrate availability. Dysregulated acetate metabolism and mitochondrial acetyl-CoA production are relevant to studies of metabolic reprogramming observed in cancer biology, insulin resistance, and other disorders characterized by impaired oxidative metabolism. ACSS1 is therefore a useful target for investigating mitochondrial substrate choice, acetyl-CoA compartmentalization, and downstream effects on redox balance and cellular bioenergetics.
ACSS1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ACSS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ACSS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ACSS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ACSS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.