Anticorps WT1 (H-1) recommandé pour la détection de WT1 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps WT1 (H-1): sc-393498

Anticorps WT1 (H-1) est évalué 3.3 de 5 de 12.
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Fiches techniques

    • L'Anticorps WT1 (H-1) est un monoclonal de souris IgM κ, cité dans 5 publications, fourni en 200 µg/ml
    • spécifique d'un épitope situé entre les acides aminés 123-164 au niveau d'une région interne de WT1 d'origine human
    • recommandé pour la détection de WT1 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA; également réactif avec d'autres espèces, dont et bovine and porcine
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de WT1 (H-1): sc-393498.
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I would like to use the WT-1 (H-1) antibody to stain for podocytes in kidney organoids. Do you have any secondary antibody to recommend for fluorescence microscopy?

Posée par: ValeM
Thank you for your question. Here is a link to our secondary antibodies: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Répondue par: Tech Service
Date de publication: 2019-05-21

Hi, can I ask for the antibody WT1 (H1) insert. Actually I'm looking for WT1 which detect carboxyl Terminus or C-Terminus. Is this is the correct one? to rule out DSRCT (Desmoplastic Small Round Cell Tumours). Can I have the sample for evaluation? TQ

Posée par: RUZA
Thank you for your question. This mouse monoclonal IgM antibody is specific for an epitope mapping between amino acids 123-164 within an internal region of WT1 of human origin. It does not specifically recognize the C-terminus. We do not currently offer any WT1 antibodies with C-terminal epitopes. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Répondue par: Technical Support
Date de publication: 2019-03-03

I am using WT1 (H-1): sc-393498 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Posée par: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6.0) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support 15
Date de publication: 2017-01-20
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Rated 1 de 5 de par 免疫組織化学でうまく機能しない 精巣のパラフィン切片を使って免疫蛍光染色を行いましたが、 セルトリ細胞の核が全く染色されませんでした。
Date de publication: 2020-12-23
Rated 5 de 5 de par Dr Sadek Ab work good with dilution 1:200 and 2 hrs incubation at 37ºc and overnight at 4 ºc
Date de publication: 2018-11-21
Rated 1 de 5 de par No IHC or IF staining at all. We acquire a sample and perform IHC and IF on 4um mouse kidney section with M.O.M. kit. None of them works. Podocytes does not have nuclear staining of WT-1, nor has cytoplasmic staining. It`s just completely negative. I really hope the santa Cruz can resume C-19 (sc-192) since it works perfectly on frozen sections with 4% PFA fixation of 20min.
Date de publication: 2018-08-23
Rated 1 de 5 de par WT1 antibody Sc-393498 Purchased this antibody recently. Tried it on WB and IP-MS experiments. Didn't work in either. :((
Date de publication: 2018-05-07
Rated 1 de 5 de par 染的都是背景 I applied a sample sack in Sep. and then I did IF in both testis and heart. Results showed that the nonspecific staining as it stained in cytoplasm and myocardiocytes where supposed not be appear.
Date de publication: 2017-10-12
Rated 5 de 5 de par Good, BUT Antibody is perfect, but it maybe unsuitable to replace C-19, because my aim is detect EWS-WT1. The service of SCBT is very nice!
Date de publication: 2017-07-31
Rated 4 de 5 de par 抗体使用评论 用这个抗体做WB实验,使用了NB4细胞系,加药处理测WT1变化。实验结果还行。但是还需要重复实验验证结果。
Date de publication: 2017-07-21
Rated 5 de 5 de par Cost-effective Our laboratory has been using your company's antibody, it is very practical!
Date de publication: 2017-07-05
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