Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Wnt-5b CRISPR/Cas9 KO Plasmid (m): sc-423719
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Wnt-5b CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Wnt-5b genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Wnt-5b Antibody (G-4): sc-376249
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Wnt-5b CRISPR/Cas9 KO Plasmid (m) | sc-423719 | 20 µg | $397.00 |
Overview
Mouse Wnt5b encodes the secreted glycoprotein Wnt-5b, a non-canonical Wnt ligand that regulates cell polarity, directional migration, and cytoskeletal remodeling during development and tissue homeostasis. Wnt-5b preferentially signals through β-catenin–independent pathways such as planar cell polarity and Wnt/Ca2+ signaling, engaging receptors including Frizzled and co-receptors such as ROR family tyrosine kinases to tune downstream JNK, PKC, and small GTPase activity. These pathways influence morphogenesis, neuronal patterning, and stromal–epithelial communication, and dysregulated WNT5B expression has been linked to altered differentiation programs and invasive cell behaviors in disease-related contexts. As a pathway node that intersects with inflammatory and extracellular matrix signaling, Wnt-5b is frequently examined in models of development, metabolism, and tumor microenvironment biology.
Wnt-5b CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Wnt5b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Wnt5b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Wnt5b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Wnt-5b protein expression.
This CRISPR knockout system enables efficient generation of Wnt5b-deficient cell models for investigation of Wnt-5b signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Wnt5b exon(s) critical for Wnt-5b function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Wnt5b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Wnt-5b CRISPR/Cas9 KO Plasmid (m) and Wnt-5b CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Wnt5b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Wnt-5b HDR Plasmid (m) and Wnt-5b HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Wnt5b homology arms to support homology-directed repair at defined Wnt5b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.