WNK1 Double Nickase Plasmid (h): sc-403420-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WNK1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WNK1 Double Nickase Plasmid (h) and WNK1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WNK1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WNK1 Double Nickase Plasmid (h) | sc-403420-NIC | 20 µg | $410.00 | |||
WNK1 Double Nickase Plasmid (h2) | sc-403420-NIC-2 | 20 µg | $410.00 |
WNK1 (with-no-lysine [K] 1) encodes a serine/threonine kinase that functions as an upstream regulator of ion transport and cell volume homeostasis through phosphorylation cascades involving SPAK/OSR1 and downstream SLC12 family cotransporters. By integrating osmotic stress cues with signaling outputs, WNK1 influences epithelial electrolyte handling, cytoskeletal dynamics, and kinase network cross-talk affecting proliferation and migration. WNK1 activity is linked to blood pressure regulation and renal salt handling, and dysregulation of WNK1-dependent pathways has been studied in contexts including hypertension-associated phenotypes and neuropathies. These features make WNK1 a useful node for dissecting osmoresponsive signaling and transport pathway regulation in human cell models.
WNK1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WNK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WNK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WNK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WNK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.